Immunohistochemical Localization of Nerve Growth Factor, Glial Fibrillary AcidicProtein and Ciliary Neurotrophic Factor in Mesencephalon, Rhombencephalon,and Spinal Cord of Developing Mongolian Gerbil

초록

The distribution of the nerve growth factor (NGF),the glial fibrillary acidic protein (GFAP) and theciliary neurotrohic factor (CNTF) was performed incoronal sections of the mesencephalon, rhombencephalonand spinal cord in the developing Mongolian gerbils.Generaly, NGF specificaly recognizes neurons withthe NGF receptor, whereas GFAP does the glia, andwas examined separately in gerbils between embryonicdays 15 (E15) and postnatal weks 3 (PNW 3). TheNGF-IR was first observed in the spinal cord at E21,which might be related to the maturation. The GFAPreactivity was peaked at he postnatal days 2 (PND2),while the highest CNTF-reaction was expresed atPNW 2. The GFAP stains were observed in theaqueduct and the spinal cord, which appeared toproject lateraly at E19. The CNTF was observed onlyafter the birth and found in both the neurons andcerebelum and the spinal cord from PND1 to PNW3.These results uggest hat NGF, GFAP and CNTF areimportant for the development of the neurons and theneuroglia in the central nervous system at he lateprenatal and postnatal stages.

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