명월초에서 분리한 Broad bean wilt virus 2 분리주들의 염기서열 분석과 기주반응 비교

초록

Gynura procumbens (Myeongwolcho or longevity spinach) showing virus-like mosaic and yellowing symptoms was collected in Chuncheon. To identify the virus from G. procumbens, RT-PCR analysis was performed using several genus-specific primers sets. BBWV2-Gyp was identified from the amplified target product using Fabavirus genus-specific primers. BBWV2- Gyp had a similar host range pattern to BBWV2–SN that has already reported in G. procumbens. BBWV2-Gyp induced systemic mosaic and BBWV2-SN induced systemic necrotic spots on Phaseolus angularis. To investigate the sequence specificity, BBWV2-Gyp RNA2 was sequenced and compared with other BBWV2 isolates. Sequence homologies and phylogenetic analyses clearly indicated that BBWV2-Gyp had the closest relationship to BBWV2-SN. Also, BBWV2- Gyp had 98.5%, 98.8% and 98.5% homologies with movement protein (MP), large coat protein (LCP) and small coat protein (SCP) segments, respectively, of BBWV2-SN at amino acid level. Although two BBWV2 isolates from G. procumbens have high nucleotide sequence similarity (99%), they have distinct biological properties.

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