Neuroprotective Diarylheptanoids from the Leaves and Twigs of Juglans sinensis against Glutamate-Induced Toxicity in HT22 Cells

초록

A new diarylheptanoid, juglanin C (1), was isolated from the 80% methanolic extract of the leaves and twigs of Juglans sinensis with three known diarylheptanoids, juglanin A (2), juglanin B (3), and (5R)-5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1(4-hydroxyphenyl)-3-heptanone (4), using bioactivity-guided fractionation and chromatographic techniques. Among the isolated diarylheptanoids, compounds 1 and 2 significantly showed neuroprotective activities against glutamate-induced toxicity in HT22 cells. These two diarylheptanoids significantly reduced the overproduction of cellular peroxide in glutamate-injured HT22 cells. Moreover, these two diarylheptanoids significantly maintained antioxidative defense systems, including glutathione, glutathione reductase, and glutathione peroxidase, under glutamate-induced oxidative stress in HT22 cells.

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