Phylogenetic relationship and evolution analysis of Citrus species

초록

The genus Citrus (Aurantioideae, Rutaceae) is the sole source of the citrus fruits for commerce showing high economic values. Due to wide dispersion, natural hybridization sensitivity and long history of cultivation, their taxonomy and phylogeny of Citrus genus have been still the research focus of taxonomists. In this study, the taxonomy and phylogeny of Citrus species are evaluated within 28 different Citrus species based on five multi-locus DNA markers. Among these markers, the nrDNA ITS and rRNA 5S showed relatively higher variation rates of sequence nucleotide, of which the rRNA 5S region exhibited the hybridization information clearly. According to the rRNA 5S and other DNA marker sequence analysis, one Citrus spp. was determined to be C. unshiu. Given consideration to high sequence conservation, the rRNA 18S and cpDNA markers were more suitable for fixing to avoid slipping of DNA sequences when aligned. Thus, we combined with all the five DNA markers and constructed one combined phylogenetic tree, that is further in favor of understanding the evolution and phylogenetic relationship of Citrus species.

키워드