Overexpression and purification of full-length acidic fibroblast growth factor in E.coli

초록

A full-length aFGF cDNA was cloned and inserted into a T7 expression vector for heterologous expression in E. coli. For high level production of aFGF, an optimal condition for overexpression (incubation at 42 °C for 2 hours) and a simple purification procedure employing in vitro refolding and an affinity chromatography was established. The production yield was 40-50 μg/mg of inclusion body preparation and purified aFGF showed mitogenic activity. © 1994 Chapman & Hall.