Genetic Diversity among Waxy Corn Inbred Lines Revealed by CACTA-TD Markers

초록

CACTA is a class 2 transposon superfamily found exclusively in plants. In this study, we have applied CACTA-TD technique to study the genetic diversity among 90 waxy corn inbred lines. While CACTA-TD fragments ranged approximately from 100 to 650 bp in size, the fragments in the range of 100-500bp showed high profiles of polymorphisms. Analysis of the 90 inbred lines with 5 CACTA-TD primer combinations detected a total of 204 fragments, of which 165 (80.9%) were polymorphic among the inbreds. The GDs of among all inbreds measured by CACTA-TD markers showed 38.8±7.6. The GDs between groups (KW vs QL) were slightly higher than the GDs within groups. GDs of KW vs QL were 42.1±5.1, while the GDs of KW vs KW and QL vs QL showed 37.0±7.6 and 38.9±10.2, respectively. In cluster analysis of the 90 waxy corn inbred lines, three major groups were recognized; Group I contained 5 KW lines, 29 QL lines and 1 HW line, with 0.65~0.75 similarity between them. Group II contained 9 KW lines, 5 QL lines and 1 HW line, with 0.62~0.87 similarity between them. Group III contained 3 KW lines and 37 QL lines, with 0.61~0.71 similarity between them. The results of current study will be utilized for the dissection of quantitative traits and the identification of superior QTLs from the waxy corn hybrid, and will be used for the further genetic analysis and maize breeding programs.

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