Effects of Rosa multiflora root extract on adipogenesis and lipogenesis in 3T3-L1 adipocytes and SD rat models

초록

BACKGROUND/OBJECTIVES: Obesity is a major cause of metabolic disorders; to prevent obesity, research is ongoing to develop natural and safe ingredients with few adverse effects. In this study, we determined the anti -obesity effects of Rosa multiflora root extract (KWFD-H01) in 3T3 -L1 adipocytes and Sprague-Dawley (SD) rats. MATERIALS/METHODS: The anti -obesity effects of KWFD-H01in 3T3 -L1 adipocytes and SD rats were examined using various assays, including Oil Red O staining, gene expression analyses, protein expression analyses, and blood biochemical analyses. RESULTS: KWFD-H01 reduced intracellular lipid accumulation and inhibited the mRNA expression of peroxisome proliferator-activated receptor gamma (PPAR gamma), cytidine-cytidineadenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins (C/EBP alpha), sterol regulatory element -binding transcription factor 1 (SREBP-1c), acetyl-CoA carboxylase (ACC), and fatty acid synthase (FAS) in 3T3 -L1 cells. KWFD-H01 also reduced body weight, weight gain, and the levels of triglycerides, total and LDL-cholesterol, glucose, and leptin, while increasing high -density lipoprotein -cholesterol and adiponectin in SD rats. PPAR gamma, C/EBP alpha, SREBP-1c, ACC, and FAS protein expression was inhibited in the epididymal fat of SD rats. CONCLUSION: Overall, these results confirm the anti -obesity effects of KWFD-H01 in 3T3 -L1 adipocytes and SD rats, indicating their potential as baseline data for developing functional health foods or pharmaceuticals to control obesity.

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