Enhancement of catharanthine production by the addition of paper pulp waste liquors to Catharanthus roseus in chemostat cultivation

초록

Dialyzed paper pulp waste liquor added at 20% (v/v) basal medium improved the production of indole alkaloid, catharanthine, from Catharanthine roseus, yielding 51 μg/cell as against 42 μg/cell in non-supplemented medium. 30-40% (v/v) of catharanthine was secreted out of the cells, showing that the change of the ratio of inner to outer concentrations was from 12.3 to 2.2 at dilution rate of O.1 h-1 during chemostat cultivation. An optimal dilution rate was 0.34 h-1 in 20% (v/v) supplemented medium, compared to 0.22 h-1 in non-supplemented medium.