Direct detection of Brenneria rubrifaciens by (polymerase chain reaction) PCR-based assay using rubrifacine synthetic gene

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초록

A rapid high-throughput, specific and sensitive (polymerase chain reaction) PCR-based assay coupled with DNA hybridization technique for the detection of deep bark canker (DBC) pathogen Brenneria rubrifaciens directly from mixed bacterial cells and in planta was developed. These result shows that the specific genetic markers have a powerful potential to detect B. rubrifaciens directly from crude samples at field conditions.

키워드

Brenneria rubrifaciensdeep bark cankerPCR (polymerase chain reaction)rubrifacineCAUSAL AGENTWALNUTCANKER
제목
Direct detection of Brenneria rubrifaciens by (polymerase chain reaction) PCR-based assay using rubrifacine synthetic gene
저자
Thapa, Shree PrasadLim, Chun KeunKim, Sam KyuCho, Jun MoHur, Jang HyunPark, Duck Hwan
발행일
2010-08-18
유형
Article
저널명
African Journal of Microbiology Research
4
16
페이지
1754 ~ 1757