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Direct detection of Brenneria rubrifaciens by (polymerase chain reaction) PCR-based assay using rubrifacine synthetic gene
- Thapa, Shree Prasad;
- Lim, Chun Keun;
- Kim, Sam Kyu;
- Cho, Jun Mo;
- Hur, Jang Hyun;
- ... Park, Duck Hwan
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3초록
A rapid high-throughput, specific and sensitive (polymerase chain reaction) PCR-based assay coupled with DNA hybridization technique for the detection of deep bark canker (DBC) pathogen Brenneria rubrifaciens directly from mixed bacterial cells and in planta was developed. These result shows that the specific genetic markers have a powerful potential to detect B. rubrifaciens directly from crude samples at field conditions.
키워드
Brenneria rubrifaciens; deep bark canker; PCR (polymerase chain reaction); rubrifacine; CAUSAL AGENT; WALNUT; CANKER
- 제목
- Direct detection of Brenneria rubrifaciens by (polymerase chain reaction) PCR-based assay using rubrifacine synthetic gene
- 저자
- Thapa, Shree Prasad; Lim, Chun Keun; Kim, Sam Kyu; Cho, Jun Mo; Hur, Jang Hyun; Park, Duck Hwan
- 발행일
- 2010-08-18
- 유형
- Article
- 권
- 4
- 호
- 16
- 페이지
- 1754 ~ 1757
- 언어
- ENG
- 출판사
- ACADEMIC JOURNALS
- 발행국가
- 나이지리아
- 분량
- 4 페이지
- ISSN
- P 1996-0808