Real-time Selective Detection of Hydrogen Peroxide Based on a Tantalum Deposited Pencil Lead Electrode for Evaluation of Enzyme Activities

초록

In this study, we have carried out electrodeposition of tantalum (Ta) nanostructures on pencil lead electrode in non-aqueous media at room temperature by applying a constant potential. The deposited Ta on pencil lead was examined for the catalytic effect regarding hydrogen peroxide (H2O2) reduction with voltammetry and amperometry. Ta/pencil lead electrode exhibited amperometric sensitivity of 0.317 mu AmM-1 cm(-2) and fast response time of 0.75s, where selective detection of H2O2 was fulfilled without interruption from common electroactive biomaterials such as O-2, uric acid, ascorbic acid, dopamine, acetamidophenol, and glucose. For practical applications, the dynamic concentration changes of H2O2 during catalase and glucose oxidase-involved reactions, either eliminating or producing H2O2, were successfully traced in real time with as-prepared electrode. From the kinetics study for catalase and glucose oxidase, we evaluated Michaelis constants (K-m(app)) as 7.8mM for catalase and 37mM for glucose oxidase, respectively.

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