Effects of Quercetin and Genistein on Boar Sperm Characteristics and Porcine IVF Embyo Developments

초록

Quercetin and genistein, plentifully present in fruits and vegetables, are flavonoid family members that haveantioxidative function and plant-derived phytoestrogen activity. The antioxidative effects of quercetin and genistein onboar sperm characteristics and in vitro development of IVF embryo were investigated. The sperm motility wasincreased by addition of genistein 50 μM for 6 hr incubation compared to control (p<0.05). The sperm viability wasincreased by addition of quercetin 1 and 50 μM and genestein 1 and 50 μM for 3 hr incubation. In addition, thesperm viability seemed to be increased dose-dependantly by addition of quercetin or genistein 1 and 50 μM,respectively (p<0.05). The membrane integrities were not increased by quercetin or genistein treatments for 3 hr or6 hr incubation period except for quercetin 1 μM for 3 hr incubation. In mitochondrial activities, addition of quercetin50 μM for 6 hr incubation increased mitochondrial activity but decreased at 100 μM concentration compared withcontrol (p<0.05). When porcine IVF embryos were cultured in PZM-3 medium supplemented with low concentrations of quercetin(1∼10 μM), the developmental rates to morula and blastocyst increased but significantly decreased at high concentrationsof quercetin (25∼50 μM). The highest developmental rate to blastocysts among all concentrations of quercetinwas shown at quercetin 10 μM (p<0.05). The developmental rates to morula or blastocysts at low (0.01∼1 μM) andhigh (5∼10 μM) concentrations of genistein were not significantly different among all treatment group and genisteindid not affect on IVF embryo development. These results suggest that quercetin and genistein seem to have positive effects at certain concentrations on spermcharacteristics such as motility, viability and mitochondrial activity. In addition, low concentrations of quercetin (1,5 and 10 μM) in this experiment, seem to have beneficial effect on porcine IVF embryo development but genisteindid not affect on it at all given concentrations (0.01∼10 μM).

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