Antioxidant effects and hepatoprotective activity of 2,5-dihydroxy-4,3′-di(β-D-glucopyranosyloxy)-trans-stilbene Morus bombycis Koidzumi roots on CCl4-induced liver damage

초록

We investigated hepatoprotective activity and antioxidant effect of the 2,5-dihydroxy-4,3'-di (beta-D-glucopyranosyloxy)-trans-stilbene that purified from Morus bombycis Koidzumi roots against CCl4-induced liver damage in rats. The 2,5-dihydroxy-4,3'-di(P-D-glucopyranosyloxy)-trans-stilbene displayed dose-dependent superoxide radical scavenging activity (IC50 = 430.2 mu g/ml), as assayed by the electron spin resonance (ESR) spin-trapping technique. The increase in aspartate aminotransferase (AST) activities in serum associated with carbon tetrachloride (CCl4)-induced liver injury was inhibited by 2,5-dihydroxy-4,3'-di(beta-D-glucopyranosyloxy)-trans-stilbene and at a dose of 400 similar to 600 mg/kg samples had hepatoprotective activity comparable to the standard agent, silymarin. The biochemical assays were confirmed by histological observations showing that the 2,5-dihydroxy-4,3'-di(beta-D-glucopyranosyloxy)-trans-stilbene decreased cell ballooning in response to CCl4 treatment. These results demonstrate that the 2,5-dihydroxy-4,3-di(beta-D-glucopyranosyloxy)-trans-stilbene is a potent aritioxidant with a liver protective action against CCl4-induced hepatotoxicity.

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