Isolation of Polysaccharides from Trichoderma harzianum with Antioxidant, Anticancer, and Enzyme Inhibition Properties

Saravanakumar, Kandasamy; Park, SeonJu; Sathiyaseelan, Anbazhagan; Mariadoss, Arokia Vijaya Anand; Park, Soyoung; Kim, Seong-Jung; Wang, Myeong-Hyeon

doi:10.3390/antiox10091372

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Isolation of Polysaccharides from Trichoderma harzianum with Antioxidant, Anticancer, and Enzyme Inhibition Properties

Saravanakumar, Kandasamy;
Park, SeonJu;
Sathiyaseelan, Anbazhagan;
Mariadoss, Arokia Vijaya Anand;
Park, Soyoung;
... Kim, Seong-Jung;
외 1명

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40

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43

초록

In this work, a total of six polysaccharides were isolated from culture filtrate (EPS1, EPS2) and mycelia (IPS1-IPS4) of Trichoderma harzianum. The HPLC analysis results showed that EPS1, EPS2, IPS1, and IPS2 were composed of mannose, ribose, glucose, galactose, and arabinose. The FT-IR, H-1, and C-13 NMR chemical shifts confirmed that the signals in EPS1 mainly consist of (1 -> 4)-linked alpha-d-glucopyranose. EPS1 and IPS1 showed a smooth and clean surface, while EPS2, IPS2, and IPS3 exhibited a microporous structure. Among polysaccharides, EPS1 displayed higher ABTS(+) (47.09 +/- 2.25% and DPPH (26.44 +/- 0.12%) scavenging activities, as well as higher alpha-amylase (69.30 +/- 1.28%) and alpha-glucosidase (68.22 +/- 0.64%) inhibition activity than the other polysaccharides. EPS1 exhibited high cytotoxicity to MDA-MB293 cells, with an IC50 of 0.437 mg/mL, and this was also confirmed by cell staining and FACS assays. These results report the physicochemical and bioactive properties of polysaccharides from T. harzianum.

키워드

antioxidant; antidiabetics; polysaccharides; MDA-MB293; cytotoxicity; Trichoderma; STRUCTURAL-CHARACTERIZATION; ALPHA-GLUCOSIDASE; EXOPOLYSACCHARIDE; PSEUDOKONINGII; EXTRACTS; OXIDASE; DEATH; CELLS

제목: Isolation of Polysaccharides from Trichoderma harzianum with Antioxidant, Anticancer, and Enzyme Inhibition Properties

저자: Saravanakumar, Kandasamy; Park, SeonJu; Sathiyaseelan, Anbazhagan; Mariadoss, Arokia Vijaya Anand; Park, Soyoung; Kim, Seong-Jung; Wang, Myeong-Hyeon

DOI: 10.3390/antiox10091372

발행일: 2021-09

유형: Article

저널명: ANTIOXIDANTS

권: 10

호: 9