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Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes
- Jamal, Mohammad Abu Hena Mostofa;
- Sharma, Satya Priya;
- Chung, Hea-Jong;
- Kim, Hyeon-Jin;
- Hong, Seong-Tshool;
- ... Lee, Seungkoo
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15초록
Current colony PCR methods are not suitable for screening genes encoded in genomic DNA and are limited to E. coli host strains. Here, we describe an ultra-high efficient colony PCR method for high throughput screening of bacterial genes embedded in the genomic DNA of any bacterial species. This new technique expands colony PCR method to several hosts as well as offers a rapid, less expensive and reliable bacterial genomic DNA extraction.
키워드
Colony PCR; DNA extraction; High throughput screening; Sonication; Sequencing; RIBOSOMAL-RNA GENE; IDENTIFICATION
- 제목
- Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes
- 저자
- Jamal, Mohammad Abu Hena Mostofa; Sharma, Satya Priya; Chung, Hea-Jong; Kim, Hyeon-Jin; Hong, Seong-Tshool; Lee, Seungkoo
- 발행일
- 2017-09
- 유형
- Article
- 권
- 57
- 호
- 3
- 페이지
- 365 ~ 369
- 언어
- ENG
- 출판사
- SPRINGER
- 발행국가
- 미국
- 분량
- 5 페이지
- ISSN
- E 0973-7715
P 0046-8991