Formation mechanism of BAMLET by 2D Raman correlation analysis

초록

A partially unfolded bovine alpha-lactalbumin (ALA) and oleic acid (OA) complex called bovine alpha-lactalbumin made lethal to tumor cells (BAMLET) is a tumoricidal complex. For the first time, this study investigated the pH-induced formation mechanism of the ALA/OA complex using Raman spectroscopy. For a deeper understanding of the formation mechanism of the ALA/OA complex with decreasing pH, principal component analysis (PCA) and two-dimensional correlation spectroscopy (2D-COS) were applied to pH-dependent Raman spectra of the ALA/OA complex. Based on the results of the PCA and 2D-COS analyses, we successfully elucidated the formation mechanism of the ALA/OA complex at the molecular level. We also confirm that the ALA/OA complex formed below pH 3.0. (C) 2018 Elsevier B.V. All rights reserved.

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