Role of Bax Inhibitor-1 (LeBI-1) Gene in Tomato Treated with Various Abiotic and Oxidative Stresses

초록

Phylogenetic analysis based on the deduced amino acid sequence of the Bax inhibitor-1 gene (LeBI-1) cDNA from tomato (Lycopersicon esculentum L.) revealed a high sequence similarity to Bax inhibitor-1 genes found in Capsicum annuum (91%), Nicotiana tabacum (87%), Brassica napus (75%), Arabidopsis thaliana (77%), and Zea mays (70%). There are multiple copies of the LeBI-1 gene throughout the tomato plant, with high levels of expression in the roots and flowers and low levels at the mature green stage and stem. Northern blotting analysis revealed that the expression of LeBI-1 was induced in response to wounding and cold but NaCl treated samples showed down-regulation. In addition, a gradual increase in expression was seen following treatment with abscisic acid and MV. An increase in expression was also seen for up to 12 h after exposure to H2O2, followed by a decrease after 24 h, after which a stable expression level was reached until 48 h. These results demonstrate that the LeBI-1 gene may play a role in tomato’s responses to abiotic stresses and be useful in improving of plant tolerance to abiotic stresses.

키워드