Simultaneous Determination of Seven Compounds Isolated from Euonymus alatus by HPLC-DAD

초록

Euonymus alatus has traditionally been utilized for its wide-ranging medicinal benefits, such as supporting cardiovascular health by preventing atherosclerosis, relieving menstrual pain, promoting blood circulation, easing pain, and dissipating blood stasis. In this research, a high-performance liquid chromatography (HPLC) technique was established to simultaneously quantify seven bioactive constituents, moretenone, moretenol, friedelanol, lupenone, β-sitosterol, betulin, and glycerol 1-tetracosanoate, isolated from E. alatus. The method underwent comprehensive validation, demonstrating strong linearity, precision, and accuracy. All analytes showed excellent linear correlation, with R² values exceeding 0.9990. The method also showed high repeatability and reproducibility, with intra-day and inter-day relative standard deviations (RSDs) ranging from 0.12% to 0.99% and 0.12% to 1.00%, respectively. Recovery tests confirmed the method's accuracy, yielding recoveries between 97.24% and 109.55%. These findings suggest that the developed HPLC method is robust and dependable, making it a valuable tool for the quality control and standardization of E. alatus preparations containing these key compounds. © 2025, Korean Society of Pharmacognosy. All rights reserved.

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