Catalase와 β-Mercaptoethanol이 돼지 태아섬유아세포 Clonal Lines의 배양에 미치는 영향

초록

This study was performed to examine the effects of catalase and β-mercaptoethanol (βME) on the establishment of clonal lines from porcine fetal fibroblast cells. Fibroblasts derived from a pig fetus (Day 50) were passaged two times before use. A single cell was seeded in 96-well plates and cultured in medium supplemented with or without catalase or βME. Cell colonies were passaged two times into 4-well dish. Cell lines with proliferating potential were classified as an established clonal cell line. In experience 1, the establishment efficiencies were examined by addition of catalase (100 ng/ml) or βME (100 uM) in culture medium. The establishment efficiency of βME-added group (8.3%) was significantly higher than that of control group (3.2%, P<0.05). However, catalase did not have a positive effect on the establishment efficiency. In experience 2, the establishment efficiencies were examined by addition of different concentrations of catalase (0~1,000 ng/ml) in culture medium. However, establishment efficiencies were not different among the different concentrations of catalase (0~2.6%). In experience 3. the establishment efficiencies were examined by addition of different concentrations of βME (0~1,000 uM) in culture medium. The establishment efficiency was significantly higher in 100 uM βME-added group (9.4%) compare to others (0~1.6%). The result of present study shows that the establishment efficiency of clonal cell lines can be enhanced by the culture in media supplemented with 100 uM βME. However, catalase did not have a positive effect on the establishment efficiency.

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