Antioxidative Activity of Prunus sargentii Outer Bark Extractives

초록

The outer bark of Prunus sargentii was collected, air-dried and extracted with 70% aqueous acetone. Then it was successively partitioned with n-hexane, dichloromethane (CH2Cl2), ethyl acetate (EtOAc) and H2O. From the EtOAc soluble fraction, four compounds were isolated by the repeated Sephadex LH-20column chromatography. The isolated compounds were determined as (+)-catechin (1), (-)-epicatechin (2),taxifolin (3), and neosakuranin (4) by the spectroscopic analysis including 1H,13C-NMR, and 2D-NMR spectrometers. The antioxidative activities on the isolated compounds and the separated fractions were evaluated by DPPH radical scavenging assay. The crude, EtOAc, and H2O soluble fractions indicated good antioxidative potential compared to the CH2Cl2 and n-hexane soluble fractions.

키워드