Effects of Superparamagnetic Iron Oxide Nanoparticles on Essential Attributes Requested in Bone Marrow-Derived Mesenchymal Stem Cells Used for Neurological Disease Therapy

초록

Superparamagnetic iron oxide (SPIO) nanoparticles are considered promising cell trackers due to their enhanced sensitivity and spatial resolution. Magnetic resonance imaging (MRI) analysis uses SPIO nanoparticles to monitor cells transplanted into the body; however, no studies have investigated their effects on the cytological safety and neurogenic differentiation of the mesenchymal stem cells (MSCs) used to treat intractable neurological diseases. Therefore, in this study, we examined the effect of SPIO on the physiological events and neurogenic differentiation potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) in rats. We analyzed cell viability, proliferation, and migration, as well as MSC marker expression and neurogenic differentiation, in BM-MSCs labeled with different concentrations of SPIO. As the results, SPIO labeling exhibited no significant influence on the viability, proliferation, migratory ability, protein expression, or neurogenic differentiation potential of MSCs. These results demonstrate that SPIO can be used alone as a tracker for BM-MSC trackers transplanted into the region of the central nervous system without inducing any cytological damage.

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