Impact of silver nanoparticles on the micropropagation of Hybanthus enneaspermus and assessment of genetic fidelity using RAPD and SCoT markers

초록

Key message The green synthesized AgNPs increased the regeneration frequency and there is no genetic variation among the invitro regenerated plants. A prompt and dynamic clonal propagation system was developed for Hybanthus enneaspermus a medicinally significant plant using shoot tip as explants. Multiple shoots were induced on Murashige and Skoog (MS) medium invigorated with different levels of 6-benzylaminopurine (BAP), kinetin (Kin) and thidiazuron (TDZ) and AgNPs. The optimum concentration of BAP (1.5 mg L-1 ) with AgNPs (3.0 mg L-1) exhibited a determined response of 90.66% and created a maximum of 77.23 shoots with a maximum shoot length of 3.72 cm after 4 weeks. The vigorous shoots were embedded on an MS medium modified with optimum concentration of IBA 1.0 mg L-1 and AgNPs 1.0 mg L-1, which engendered the highest number of roots (9.65) with an average root length of 4.77 cm, with the response of 87%. Further, rooted plantlets were efficiently acclimatized in the soil. In addition, genetic homogeneity analysis was performed using RAPD and SCoT molecular marker, which revealed that the regenerated plants were genetically uniform and no detectable genetic difference was observed when compared to the mother plant. This efficient micropropagation protocol provides a new way for targeted gene editing for functional analysis of genes in Hybanthus enneaspermus.

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