A new method for microbial cultivation and its application to bacterial community analysis in Buus Nuur, Mongolia

초록

We performed a comparative analysis of bacterial diversity in a saline soda lake using a novel cultivation method termed the filter plate microbial trap method (FPMT), and conventional techniques together with DGGE (denaturing gradient gel electrophoresis). The new method allowed for cultivation of representatives of seven bacterial taxonomic groups, including seven novel species. This compares favorably to the results from cultivation of representatives of four taxonomic groups and only one novel species using standard approaches. Neither culture collection matched the community composition revealed by DGGE but the FPMT method produced a more representative collection of strains. We conclude that FPMT is a powerful device for improving bacterial culturability and narrowing the gap between microbial diversity in nature and cultivable microorganisms.

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