Comparative Studies of the Hanabusaya asiatica and its Allied Groups by RAPD Analysis

초록

The phylogenetic relationships between Hanabusaya asiatica and its allied groups were assessed by Randomly Amplified Polymorphic DNA (RAPD) analysis of genomic DNA, and their taxonomic status was reevaluated at the molecular level. The analyzed plants consisted of 36 populations of eight genera and 27 taxa. Forty-two out of 101 primers(10-mer) screened were able to amplify DNA. Only 19 primers, however, actually succeeded in DNA amplification, resulting in 523 randomly amplified DNA fragments. The analyzed taxa showed very high polymorphism, allowing each individual taxon to be identified based on RAPD analysis. All eight genuses were differentiated from each other at the 0.86 level of similarity index value. In particular, Hanabusaya asiatica was distinguished from its closely related genus. Intraspecific and interspecific relationships were quite close, at levels ranging from 0.77 to 0.99. Our RAPD analysis supports the previous data based on morphological and palynological studies.

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