New Device for Addition of Modifier to Supercritical Fluid Carbon Dioxide Mobile Phase

초록

A new device to accurately deliver small amount of modifier into supercritical carbon dioxide fluid is described. Carbon dioxide, the most widely used mobile phase in supercritical fluid chromatography, is a relatively non-polar fluid, and hence the addition of small amount of polar modifiers could be necessary to migrate polar solutes. In this work, supercritical CO<inf>2</inf> and modifier are delivered from the pump to a 100 μL mixing chamber in which a small magnetic bar is rotating. After passing through the mixing chamber, supercritical CO<inf>2</inf> is changed to a new mobile phase with different polarity. The amount of modifier added into supercritical CO<inf>2</inf> is measured by an amperometric microsensor, which is prepared from a thin film of perfluorosulfonate ionomer.