Complete genome sequences of three bean common mosaic virus isolates identified from peanut (Arachis hypogaea L.)

초록

To identify viruses infecting peanut (Arachis hypogaea L.) plants, we collected leaf samples from three peanut plants showing viral disease symptoms in Jeonju, Korea. After extracting total RNA from the individual plant samples, we generated three different ribosome-deleted libraries for RNAsequencing. The three libraries were paired-end sequenced, and the raw data were used for de novo transcriptome assembly followed by a BLASTN search against a viral genome database. We only identified bean common mosaic virus (BCMV) infecting all three peanut plants. From the three libraries, we obtained three complete genomes of BCMV 10,049 nucleotides to 10,039 nucleotides in length encoding two open reading frames. All three BCMV complete genomes showed sequence similarity to the known BCMV isolate Habin1 identified from soybean in Korea. This result suggests that BCMV infecting peanut might originate from BCMV infecting soybean or vice versa. Taken together, we report complete genome sequences of three BCMV isolates identified from peanut in Korea.

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