Carboxylate-modified Latex Bead Surface for Analysis of Blood Proteins

초록

We developed a new carboxylate-modified latex bead surface for determining the quantitative level of blood proteins. In this study, antibody arrays are fabricated by immobilizing a target protein-specific antibody on a carboxylate-modified latex bead surface of well-type arrays. C-reactive protein (CRP) carcinoembryonic antigen, and prostate-specific antigen were conjugated with fluorophores for use as internal standards for the analysis of blood proteins. We found that the carboxylate-modified latex bead surface had a higher binding affinity for Alexa 546-CRP than dextran, aldehyde, and amine surfaces. The carboxylate-modified latex bead surface was found to be specific and reproducible. In addition, the antibody arrays were successfully applied to the analysis of CRP, carcinoembryonic antigen, and prostate-specific antigen in normal human serum (r(2)=0.997, 0.988 and 0.999, respectively). Thus, an antibody array based on a carboxylate-modified latex bead surface has a strong potential for a rapid serodiagnosis of human diseases.

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