Influence of sucrose on rutin content and flavonoid biosynthetic gene expression in seedlings of common buckwheat (Fagopyrum esculentum Moench)

초록

Rutin is the main flavonoid compound in common buckwheat (Fagopyrum esculentum Moench) and sucrose is a major enhancer of both phenolic production and organ development in plants. In this work, we measured the effect of sucrose on on the growth of buckwheat seedlings. The accumulation of rutin in these seedlings and the expression pattern of the structural genes that are involved in the flavonoid biosynthetic pathway were also investigated. The growth of buckwheat was inhibited when the concentration of sucrose was increased to 50 g/L; however, the expression of most flavonoid biosynthetic genes were increased after 1 or 2 days of treatment and rutin content showed a marked increase when the concentration of sucrose was increased from 10 g/L to 50 g/L.

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