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Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells
- Park, Hankum;
- Koo, Ja Young;
- Srikanth, Yellamelli V. V.;
- Oh, Sangmi;
- Lee, Jiyoon;
- ... Park, Jongmin;
- 외 1명
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31초록
Herein we report molecular shape-dependent nonspecific labeling of photoaffinity linkers (PLs) in the cellular proteome. Linear PLs have a greater tendency to engage in nonspecific binding than branched PLs. Exploiting this property, we discovered a smaller branched diazirine-based PL as the best photoaffinity probe with minimal nonspecific binding characteristics from among 5 probes with different PLs.
키워드
PHOTO-CROSS-LINKERS; TARGET IDENTIFICATION; BINDING PROTEINS; PROBES; DISCOVERY
- 제목
- Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells
- 저자
- Park, Hankum; Koo, Ja Young; Srikanth, Yellamelli V. V.; Oh, Sangmi; Lee, Jiyoon; Park, Jongmin; Park, Seung Bum
- 발행일
- 2016
- 유형
- Article
- 권
- 52
- 호
- 34
- 페이지
- 5828 ~ 5831
- 언어
- ENG
- 출판사
- ROYAL SOC CHEMISTRY
- 발행국가
- 영국
- 분량
- 4 페이지
- ISSN
- E 1364-548X
P 1359-7345