Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells

Park, Hankum; Koo, Ja Young; Srikanth, Yellamelli V. V.; Oh, Sangmi; Lee, Jiyoon; Park, Jongmin; Park, Seung Bum

doi:10.1039/c6cc01426g

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Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells

Park, Hankum;
Koo, Ja Young;
Srikanth, Yellamelli V. V.;
Oh, Sangmi;
Lee, Jiyoon;
... Park, Jongmin;
외 1명

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초록

Herein we report molecular shape-dependent nonspecific labeling of photoaffinity linkers (PLs) in the cellular proteome. Linear PLs have a greater tendency to engage in nonspecific binding than branched PLs. Exploiting this property, we discovered a smaller branched diazirine-based PL as the best photoaffinity probe with minimal nonspecific binding characteristics from among 5 probes with different PLs.

키워드

PHOTO-CROSS-LINKERS; TARGET IDENTIFICATION; BINDING PROTEINS; PROBES; DISCOVERY

제목: Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells

저자: Park, Hankum; Koo, Ja Young; Srikanth, Yellamelli V. V.; Oh, Sangmi; Lee, Jiyoon; Park, Jongmin; Park, Seung Bum

DOI: 10.1039/c6cc01426g

발행일: 2016

유형: Article

저널명: Chemical Communications

권: 52

호: 34

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