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초록
An efficient transformation protocol was established for Chinese foxglove, Rehmannia glutinosa L., using Agrobacterium tumefaciens. Putative transgenic plants were induced from leaf explants co-cultivated with Agrobacterium. Detection of the neomycin phosphotransferase gene, high activity of β-glucuronidase (GUS) transcripts and histochemical localization of GUS confin-ned the integrative transformation of Chinese foxglove. This result demonstrates the potential for using A. tumefaciens to transfer important genes into commercial Chinese foxglove cultivars.
키워드
Agrobacterium; Chinese foxglove; Genetic transformation; Rehmannia glutinosa L.; Shoot organogenesis
- 제목
- Agrobacterium-mediated genetic transformation of Chinese foxglove, Rehmannia glutinosa L.
- 저자
- Park, Sang-un; Kim, Haeng-hoon; Yu, Changyeon; Park, Cheol-ho; Chae, Young-am
- 발행일
- 2002
- 유형
- Article
- 권
- 24
- 호
- 7
- 페이지
- 547 ~ 550