Enhancement of sepiapterin production in recombinant Escherichia coli by coexpression of the genes for guanosine triphosphate(GTP) biosynthesis

초록

Sepiapterin, a precursor for tetrahydrobiopterin, is produced in higher mammals using guanosine triphosphate (GTP) as a biosynthetic intermediate. Four genes involved in GTP biosynthesis, namely those of guanosine monophosphate kinase (gmk), nucleoside diphosphate kinase (ndk), guanosine phosphate synthetase (guaA), and inosine-5'-monophosphate dehydrogenase (guaB), were expressed in sepiapterin-producing recombinant Escherichia coli BL2l(DE3) to increase intracellular GTP concentration and to improve sepiapterin production concomitantly. Coexpression of gmk, ndk, guaA, and guaB, doubled the intracellular GTP concentration and increased the maximum sepiapterin concentration up to 126.1 ± 19.3 mg/1 (an increase of 43% compared with control cells) in batch-cultivated recombinant E. coli. © 2016, The Korean Society for Microbiology and Biotechnology.

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