Synthesis of fluorescent dye-embedded silica nanoparticles for vitamin D3 detection using sandwich-like assay

초록

This study devised and examined a sandwich-like assay for detecting vitamin D-3 dissolved in acetonitrile using fluorescent dye-embedded silica nanoparticles (AF647@SiNPs). First, AF647@SiNPs were synthesized via the modified Stober method to embed the fluorescent dye molecules in the silicate network. Then, vitamin D-3 (sample) was immobilized using an azide linker on the magnetic nanoparticles (MNPs). The immobilized vitamin D-3 reacted with the antibody immobilized on the surface of AF647@SiNPs (Anti-VD3@AF647@SiNPs). The vitamin D-3 concentration was calculated back using the fluorescence of the remaining unreacted Anti-VD3@AF647@SiNPs. Finally, we examined how applying conditions of AF647@SiNPs influenced the measurement reliability: (1) the initial loading of AF647@SiNPs; (2) the antibody-labeling degree of AF647@SiNPs.

키워드