동과 추출물(HR1901-W)의 지표성분 2-Furoic acid 분석법 개발 및 검증

초록

As interest and demand for functional foods increase, standardization becomes important to manage the quality of raw materials and products. Analytical methods for the standardization of bioactive compounds must be created in tandem with the development of functional foods. Therefore, the aim of this study was to validate an analytical method for the determination of 2-furoic acid in Benincasa hispida extract (HR1901-W). The 2-furoic acid analysis method was validated by evaluating the linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ), using a high-performance liquid chromatography－photodiode array spectrum. Our results showed that the correlation coefficients of the calibration curve for 2-furoic acid was 0.9999. Furthermore, the LOD and LOQ for 2-furoic acid were 0.10 μg/mL and 0.32 μg/mL, respectively. The range of inter-day and intra-day precision values (RSDs, relative standard deviation) for 2-furoic acid were 0.13%－0.56% and 0.27%－0.33, respectively, and the inter-day and intra-day accuracy values (recovery) were 98.47%－102.74% and 98.77%－ 102.72%, respectively. In addition, the content of 2-furoic acid in HR1901-W was found to be 412.39 ± 0.91 μg/dry weight in grams. These results indicate that the analytical method used in this study should be appropriate for the quantitative analysis of 2-furoic acid in HR1901-W and considered for use as basic data for the standardization of HR1901-W.

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