열처리한 양파껍질 추출물이 3T3-L1 지방세포 분화와 지방축적 관련 유전자 발현에 미치는 영향

초록

Purpose: This study was conducted to investigate the effect of onion peel extract heated at various temperatures on inhibition of fat accumulation during 3T3-L1 adipocyte differentiation. Methods: Onion peel were heated at 0(OPE), 140(140H-OPE), 170(170H-OPE), and 200(200H-OPE)℃ and extracted with 70% ethanol. Fat accumulation in 3T3-L1 adipocyte was observed by Oil red O staining. Expression of fat accumulation-related genes was analyzed by real-time PCR, and protein expression was determined using Western blot. Results: As a result of Oil red O staining in 3T3-L1 adipocyte, 50 μg/mL OPE, 140H-OPE, 170H-OPE, 200H-OPE treated group was significantly reduced by 21.77, 73.08, 79.00, and 79.66%, respectively, compared to adipocyte group(p<0.05). Moreover, 50 μg/mL 170H-OPE treatment of 3T3-L1 adipocyte significantly decreased the expression level of the adipogenic transcription factor gene including pparγ, c/ebpα, and srebp-1c by 55.94, 84.85, and 67.36% respectively. In addition, those of the level of lipogenic genes such as acc1, fas, scd1, ap2, and cd36 reduced significantly by 64.79, 72.95, 94.57, 53.33, and 70.54%, respectively compared to the adipocyte control, respectively(p<0.05). Conclusion: These results imply that heated onion peel extract is a functional natural ingredient possessing potential inhibitory fat accumulation.

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