Antiproliferative Triterpenes from the Leaves and Twigs of Juglans sinensis on HSC-T6 Cells

초록

Bioassay guided fractionation of an 80% MeOH extract of leaves and twigs of Juglan sinensis has resulted in the isolation of four new triterpenes (174) and 17 known triterpenes (5721). The new compounds were determined to be 1-oxo-3 beta,23-dihydroxyolean-12en-28-oic acid 28-O-beta-D-glucopyranoside (1), 1-oxo-3 beta-hydroxyolean-18-ene (2), 3 beta,23-dihydroxyurs-12-eh-28-oic acid 28-O-beta-D-glucopyranoside (3), and beta,22 alpha-dihydroxyurs-12-en-28-oic acid 28-O-beta-D-glucopyranoside (4) by spectroscopic analysis. Compounds 2, 13, 15, and 21 showed antiproliferative activities (14.2, 14.8, 15.6, and 11.0% at 100 mu M, respectively) in HSC-T6 cells. Flow cytometry assays revealed that these compounds inhibited HSC-76 proliferation by inducing apoptosis.

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