In Vitro Culture of Primary Testicular Stromal Cells derived from Mouse with Different Genetic Background : Optimization of Culture Temperature

초록

Spermatogonial stem cells (SSCs) developed into sperms through spermatogenesis have been utilized as a usefultool in the field of regenerative medicine and infertility. However, a small number of highly qualified SSCs are residedin the seminiferous tubule of testis, resulted in developing effective in-vitro culture system of SSCs for solving simultaneouslyquantitative and qualitative problems. Presently, SSCs can be enriched on testicular stromal cells (TSCs),but there are no systematic researches about TSC culture. Therefore, we tried to optimize culture condition of TSCsderived from mouse with different strains. For these, proliferation and viability were measured and compared byculturing ICR outbred or DBA/2 inbred mouse-derived TSCs at 35 or 37℃. In case of ICR strain, primary TSCscultured at 37℃ showed significantly higher proliferation and viability than those at 35℃ and significant increase ofproliferation and viability in sub-passaged TSCs was detected in the 35℃ culture condition. Moreover, sub-passageof primary TSCs at 35℃ induced no significant effects on proliferation and viability. In contrast, in case of DBA/2strain, significantly improved proliferation were detected in the primary TSCs cultured at 35℃, which showed nosignificant difference in the viability, compared to those at 37℃. Furthermore, sub-passaged TSCs cultured at 37℃showed no significant differences in proliferation and viability, compared to those at 35℃. However, with significantdecrease of proliferation induced by sub-passage of primary TSCs at 35℃, no significant effects on proliferation andviability were resulted from sub-passage of primary TSCs at 37℃. From these results, culture temperature of primaryTSCs derived from outbred and inbred strain of mouse could be separately optimized in primary culture and subculture

키워드