Advances in Nanoparticles as Non-Viral Vectors for Efficient Delivery of CRISPR/Cas9

Kim, Minse; Hwang, Youngwoo; Lim, Seongyu; Jang, Hyeon-Ki; Kim, Hyun-Ouk

doi:10.3390/pharmaceutics16091197

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Advances in Nanoparticles as Non-Viral Vectors for Efficient Delivery of CRISPR/Cas9

Kim, Minse;
Hwang, Youngwoo;
Lim, Seongyu;
Jang, Hyeon-Ki;
Kim, Hyun-Ouk

Citations

WEB OF SCIENCE

61

Citations

SCOPUS

79

초록

The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system is a gene-editing technology. Nanoparticle delivery systems have attracted attention because of the limitations of conventional viral vectors. In this review, we assess the efficiency of various nanoparticles, including lipid-based, polymer-based, inorganic, and extracellular vesicle-based systems, as non-viral vectors for CRISPR/Cas9 delivery. We discuss their advantages, limitations, and current challenges. By summarizing recent advancements and highlighting key strategies, this review aims to provide a comprehensive overview of the role of non-viral delivery systems in advancing CRISPR/Cas9 technology for clinical applications and gene therapy.

키워드

CRISPR/Cas9; delivery; nanoparticle; non-viral vector; viral vector; BIOMEDICAL APPLICATIONS; GOLD NANOPARTICLES; BETA-THALASSEMIA; GENOME; CAS9; CRISPR-CAS9; RNA; RIBONUCLEOPROTEIN; SYSTEM; HEMOGLOBIN

제목: Advances in Nanoparticles as Non-Viral Vectors for Efficient Delivery of CRISPR/Cas9

저자: Kim, Minse; Hwang, Youngwoo; Lim, Seongyu; Jang, Hyeon-Ki; Kim, Hyun-Ouk

DOI: 10.3390/pharmaceutics16091197

발행일: 2024-09

유형: Review

저널명: Pharmaceutics

권: 16

호: 9

언어: ENG

출판사: MDPI

발행국가: 스위스

ISSN: E 1999-4923