말채나무 추출물의 α-amylase 저해 활성

초록

α-Amylase inhibitor is used to control blood glucose level by inhibiting starch digestion in the small intestine and delaying the absorption of glucose. In this study, we investigated the inhibitory effect of the ethanol extracts from more than 1400 species of plants against α-amylase with the aim of developing a new α-amylase inhibitor. In the results, Cornus walteri extracts showed the highest inhibition activity. The inhibitory effect of Cornus walteri extract on the carbohydrate hydrolysis enzymes has different sensitivities against α-amylase from salivary and pancreatin and against α-glucosidase from yeast and porcine small intestine. In the study of inhibition kinetics of α-amylase and α-glucosidase, Cornus walteri extract showed competitive inhibition against salivary and pancreatin while showing the combination of uncompetitive and noncompetitive inhibition against α-glucosidase. The Cornus walteri extract was stable at acidic and thermal conditions. As for the blood glucose and body weight levels of Cornus walteri extract, we confirmed anti-hyperglycemic and anti-obesity effects. Also, in the investigation of the mRNA lever, Cornus walteri extract upregulated the level of GLUT4 mRNA in the quadriceps muscle.

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