Growth Inhibition of the Yeast Transformant by the Expression of a Chitinase from Coprinellus congregatus

초록

Coprinellus congregatus generates several chitinases during its entire life cycle: at the growing hyphal stage and at the mushroom autolysis stage. We have isolated a chitinase gene (chi1) from the mushroom tissue at the autolysing stage, and constructed a chitinase expression vector to get large amount of enzyme protein. Chitinase 1 (chi1 cDNA was heterologously expressed in Saccharomyces cerevisiae by gall promoter. The transformants showed no specific change in growth characteristics under normal growth conditions. However the expression of the gene by the gall promoter in the yeast transformants resulted in complete growth inhibition, while laccase expression by the gall promoter showed normal growth. The chitinase activities from the transformants were also more than 3 times higher than that of the recipient strain, and the chitinase expression by the real time-PCR also showed increased expression of the chil in the yeast transformant. Expression of a chitinase which was produced at the mushroom autolysing stage of C. congregatus resulted in yeast growth inhibition.

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