Genetic diversity and relationships among cultivated types of Perilla frutescens and their weedy types in Korea and Japan

초록

Perilla frutescens Britt. (Labiatae) is extensively cultivated and used as a vegetable and oil crop in Korea and Japan. This study sought to determine the genetic diversity and relationships among cultivated types of Perilla frutescens and their weedy types in Korea and Japan. By analyzing 43 Perilla accessions from Korea and Japan using eight AFLP primer combinations, a total of 155 fragments were identified, of which 89 (57%) were polymorphic at the species level. Shannon's diversity indices, Hs, of AFLP variations for the cultivated type of var. frutescens, the weedy type of var. frutescens, the cultivated type of var. crispa and the weedy type of var. crispa were 0.93, 2.47, 1.53 and 1.66, respectively. The weedy type of var. frutescens exhibited the highest variation. In addition, Korean accessions of cultivated and weedy types of var. frutescens exhibited much higher polymorphisms than Japanese accessions; whereas Japanese accessions of cultivated var. crispa exhibited higher polymorphisms than Korean accessions of weedy var. crispa. In the phylogentic tree, two major clusters were recognized: (1) cultivated type of var. frutescens, and (2) weedy type of var. frutescens, cultivated and weedy types of var. crispa. While the cultivated types of var. frutescens and of var. crispa were sharply separated by AFLPs, the weedy type of var. frutescens and the cultivated and weedy types of var. crispa did not form distinct groups. This study's assessment of genetic diversity among the genetic resources of the two cultivated types of P. frutescens and their weedy types in this study will be helpful to the activities and future studies of Perilla breeding programs.

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