Investigation of Phenotypic and Genetic Variation in Colletotrichum acutatum Isolates in South Korea

초록

Colletotrichum acutatum J. H. Simmonds is an important fungal pathogen that causesanthracnose disease in crops. In this study, 59 C. acutatum isolates from regions in SouthKorea were collected and phenotypically compared. The average colony diameters of isolatesfrom Gyeongsangbuk-do, Chungcheongbuk-do, and Gangwon-do were 4.36 cm, 4.24 cm, and4.38 cm, respectively, following 6 days of growth on V8 juice agar. The average conidiumconcentrations of the isolates from each province were 14.43, 12.83, and 10.17 × 104/mL after 7days of growth on V8A. Amplified fragment length polymorphism(AFLP) analysis using threeprimer pairs identified 5 polymorphic bands and 56 monomorphic bands. Two polymorphicbands were identified using the E-ACC/M-CTT primer pair, one polymorphic band wasidentified using the E-ACG/M-CTG primer pair, and two polymorphic bands were identifiedusing the E-ACG/M-CTT primer pair. A dendrogram was constructed using the AFLP data, andfour clusters were identified.

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