Haploid plant production through anther culture in day-neutral strawberry (Fragaria X ananassa Duch) CV. albion

초록

The overall goal of this study is to develop an anther culture system to produce haploid plant of day-neutral strawberry (Fragaria x ananassa Duch) cv. 'Albion' for use in breeding program. This study was conducted to optimize the effect of plant growth regulators (PGRs) on callus induction and plant regeneration. Moreover, we investigated the influence of three different callus age (1-month-old, 2-month-old, and 3-month-old) and three media (MS, B5, and H1) on plantlets induction. Anthers containing microspores at the uninucleate stage were produced the highest callus formation frequency (70.0%) on Dumas De Vaulx medium (C - medium) containing 0.4 mg L-1 benzyl adenine (BA), 0.1 mg L-1 3-indole acetic acid (IAA), and 2.0 mg L-1 2,4-Dichlorophenoxyacetic acid (2,4-D). The combination of 1 mg L-1 BA and 2 mg L-1 IAA in MS medium showed highest shoot regeneration (8.1%). Finally, 2-month-old callus cultured on B5 medium containing IAA and BA gave the highest regeneration rate (16.0%) and the roots were fully developed on hormone-free B5 medium after 2 months of culture. Cytological analysis of 33 regenerants showed that 6.0% were haploid (4x = 28 chromosomes), but 3.0% hexaploid (6x = 42), 69.7% octoploid (8x = 56), 6.1% doubled-octoploid (16x = 112) and 15.2% aneuploid plants were also determined.

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