Development of a multi-responsive nanocarrier based on liposome for anti-cancer drug delivery

초록

In this study, EPC-based liposomes (L/Pol/HmG/Lum) responsive to glucose, pH, and temperature were developed by incorporating poly(dimethylaminoethyl methacrylate-co-methacrylic acid-co-hexyl methacrylate) (P(D-M-H)), hydrophobically modified glucose oxidase (HmGOD), and luminol into the liposomal structure. FT-IR spectroscopy and surface tension measurements confirmed the successful hydrophobic modification of GOD. The synthesized P (D-M-H) exhibited a lower critical solution temperature (LCST) close to physiological temperature (similar to 38 degrees C), along with pH sensitivity. The resulting liposomes displayed a multi-layered vesicle structure with a diameter of below 200 nm, a PDI of approximately 0.2, and a fluorescence quenching% of approximately 65 %. Owing to the integrated multi-stimuli-responsive components, the liposomes enabled controlled drug release under acidic pH, elevated glucose concentrations, and increased temperature. In vitro evaluations demonstrated excellent biocompatibility and low cytotoxicity (>80 % cell viability at 2 mg/mL), while significantly enhancing anti-cancer efficacy (45.0 % cell viability at 10 mu g/mL) and cellular uptake compared to free drug formulations. Furthermore, the liposomes maintained structural and functional stability during storage at 4 degrees C for up to 28 days, with no significant changes in EE%, size, or PDI.

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