Distribution of MITE Transposons in a Rice Genetic Map

초록

Molecular genetic markers have wide applicability for a various genetic analyses, and genetic mapping with PCR-based markers has identified many loci in the rice genome. This study was conducted to develop a genetic map of rice based on SSR and MITE-AFLP markers. The F2 mapping population was established from a cross between Oryza sativa var. Ilpoombyeo (Japonica type variety) and O. rufipogon W259. A total of 334 markers, including 54 SSR and 280 MITE-AFLP markers, were mapped on rice chromosomes using an F2 population. Of these, 280 markers, including 226 MITE-AFLP and 54 SSR markers were found to be genetically linked to form 12 linkage groups. The size of framework map spanned 2899.7 cM of the 12 linkage groups. The average linkage distance between markers among all linkage groups was 10.4 cM. The number of markers per linkage group ranged from 15 to 37. Most of MITE-AFLP markers were well distributed through the twelve rice chromosomes. In this study, we have exploited the MITE-AFLP markers to develop a new class of molecular markers for rice genome study. These new markers are expected to provide valuable resources for map-based studies, such as marker-assisted selection, gene tagging, and the analysis of quantitative trait loci (QTLs) in rice genome research program.

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