Inhibitory Activities of Red Ginseng Acidic Polysaccharide in Platelet Aggregation

초록

Red ginseng acidic polysaccharide (RGAP), isolated from Korean red ginseng (Panax ginseng C.A. Meyer), has been shown to have a variety of biological functions such as immunostimulating and anti-tumor activities. In the present study, we investigated whether RGAP inhibited ligand-induced platelet aggregation. The washed platelet-rich plasma was prepared from male SD rats with successive centrifugation. The platelets (108/ml) were preincubated with 1 mM of CaCl2 for 2 min either in the presence or in the absence of RGAP (10 ~ 50 g/ml) and were stimulated with collagen (2.5 g/ml) and thrombin (0.1 U/ml). RGAP dose-dependently inhibited thrombin-induced platelet aggregation with IC50 value of 26.2 ± 2.0 g/ml. In collagen-induced platelet aggregation, RGAP inhibited the reaction with an IC50 value of 31.5 ± 3.0 g/ml. RGAP potently suppressed the intracellular calcium ion, which was stimulated by thrombin (0.1 U/ml). Among mitogen-activated protein kinase (MAPK) subtypes, the extracellular signal-regulated kinase (ERK) 1/2 and p38 MAPK were analyzed in the present study. RGAP inhibited the phosphorylation of ERK2 and p38 MAPK, which was activated by collagen (2.5 g/ml). Finally, these results suggested that besides saponin fraction, RGAP take an important role in the preventive effect of Korean red ginseng against cardiovascular disease such as thrombosis and atherosclerosis.

키워드