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Functional expression and production of human H-ferritin in Pichia pastoris
- Lee, Junglim;
- Song, Hyung-seok;
- Kim, Hyun-joong;
- Park, Jong-hwan;
- Chung, Dae-kyun;
- ... Jeoung, Dooil I.;
- 외 2명
Citations
SCOPUS
15초록
Human heavy chain ferritin (H-ferritin) was cloned from human heart cDNA library and expressed in Pichia pastoris. The H-ferritin transformant was cultivated by fed-batch and the cell mass reached about 52 g cell dry wt 1-1 after 150 h. In atomic absorption spectrometry analysis, intracellular content of iron in H-ferritin transformant was measured to 3038 ± 72 μg g-1 which was 9.6-fold more than that of control strain.
키워드
Fed batch; Functional expression; Human H-ferritin; Iron; Pichia pastoris
- 제목
- Functional expression and production of human H-ferritin in Pichia pastoris
- 저자
- Lee, Junglim; Song, Hyung-seok; Kim, Hyun-joong; Park, Jong-hwan; Chung, Dae-kyun; Park, Cheonseok; Jeoung, Dooil I.; Kim, Hae-yeong
- 발행일
- 2003
- 유형
- Article
- 권
- 25
- 호
- 13
- 페이지
- 1019 ~ 1023