Native environments, structures of flavonoids, plant regeneration and multiplication, and phylogenetic relationships in Allium victorialis var. platyphyllum makino

초록

Populations of Allium victorialis var. platyphyllum were mainly located at 1,200-1,400m above sea level, especially with 10-35 degrees of slope in nearby ridges of Mt. Horyeong and Mt. Dongdae. in South Korea. (A. victorialis) P. plants grown in relatively cool (8.2-20.1 C degrees) and humid (71-74%) conditions. High levels of organic matter (O.M. 11.6-13.5%) and Ca were found in the soil of native habitats with low acidity. Two flavonoids and one streoidal saponin were isolated from bulbs. Two falvonoids were identified astragalin and kaempferol-3,4'-di-O-B-D-glucopyranoside. Primordial shoots were induced when the shoot tip and bulb explants were placed on the MS basal medium supplemented with 0.2 mg/L NAA in combination of 1.0, 2.0 mg/L BAP or zeatin. Allium victorialis grown in the Jirisan area showed very high multiplication rates with a maximum number of 98 shoots from a single shoot tip. The intraspecific variations among (Allium victorialis) P.populations including three habitats and 41 individuals based on the amplified DNA polymorphisms derived from PCR (polymerase chain reaction) method were examined. This analysis clearly classified (Allium victorialis) P. populations into three major groups; Ulleungdo, Jirisan and Odaesan populations. Each population was well distinguished by similarity index value of 0.82, but in the 15 individuals of the Ulleungdo population, individual variations were found to be very high with a similarity index value of 0.92. Individuals of the Ulleungdo population were distinguished from the other two populations by external morphological characters, such as plant height, length and width of leaf, and bulb color.

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