Purification and characterization of laccase isozymes from the white-rot basidiomycete Ganoderma lucidum

초록

Ganoderma lucidum, a medicinal white-rot basidiomycete, produces many laccase isozymes in liquid culture. Three laccase isozymes (GaLc 1, 2, 3) have been purified 32.4-fold from the crude enzyme protein through anion exchange chromatography, preparative gel electrophoresis, and electroelution. Their estimated molecular weights are 65-68 kDa, and they contain 7-10% N-linked carbohydrates. The three isozymes have identical N-terminal amino acid sequences: G-I-G-P-T. The optimum pH and temperature both for each isozyme singly and the isozyme mixture are pH 3.5 and 20°C, respectively. One isozyme (GaLc 3) is quite stable at pH 4.0-10.0, and shows good stability when incubated at temperatures lower than 40°C. The K<inf>m</inf> values of GaLc 3 for o-tolidine and 2,2′-azino-bis-(3-ethylthiazoline-6-sulfonate) (ABTS) are 401.6 μM and 3.7 μM respectively, and the V<inf>max</inf> of GaLc 3 for these substrates is 0.0198 OD min-1unit-1 and 0.0142 OD min-1unit-1, respectively.