Biological Activity of Supercritical Fluid Extracts from the Braches of Alnus japonica

초록

The branches of Alnus japonica have been used inoriental traditional medicine practice for the formulation ofmedicines as the remedies for fever, hemorrhage, diarrhea,gastroenteric disorder, lymphatic disease, and cancers. In thisstudy, the anti-bacterial, anti-oxidant, anti-inflammatory, andanti-allergic activities of a supercritical extract of branches ofAlnus japonica (AJSCF) were investigated by in vitro assays. To investigate the antioxidant and anti-inflammatory effectsof a supercritical extract of branches of AJSCF, ABTS radicalscavenging activities and inhibitory activity against nitricoxide production in lipopolysaccharide (LPS) stimulated RAW264.7 cells were examined. Furthermore, anti-bacterial activitieswere examined. AJSCF on immunoglobulin E (IgE)-induced allergic inflammation in RBL-2H3 cells. In conclusion,it was observed that dose-dependent increase in AJSCFconcentration led to an increase in the ABTS radical scavengingactivity. The AJSCF showed low cytotoxicity at 50ug/mLconcentration, which was the highest concentration of theexamination. The cells stimulated with LPS produced moreNO than normal control cells; however, cells treated withsupercritical fluid extracts of branches of Alnus japonicainhibited NO production in a concentration-dependent manner. In addition, AJSCF showed the highest antifungal activityand concentration-dependent anti-allergic activity. Theresults suggest that AJSCF might be developed as a potentanti-fungal, anti-oxidant, anti-inflammatory, and anti-allergicagent for the production of novel and functional cosmeticmaterials.

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