음나무 내피 추출물의 항돌연변이원성 및 세포독성 효과

초록

This study was performed to determine the antimutagenic and cytotoxic effect of Kalopanacis cortex endothelium. Methanol extract was used on Salmonella typhimurium TA98, TA100 and three cancer cell lines. In the Ames test, methanol extract of Kalopanacis cortex endothelium alone did not exhibit any mutagenicity but whowed substantial inhibitory effects against mutation induced by 4-nitroquinoline-1-oxide(4NQO), N'-methyl- N'-nitro-N-nitrosoguanidine(MNNG) and benzo(a)pyrene(B(a)P). The methanol extract of Kalopanacis cortex endothelium showed approximately 79.29% AND 75.38% inhibitory effect on the mutagenesis induced by 4NQO, and B(a)P, respectively, against TA98 strain. Whereas 79.49%, 89.3% and 68.85% inhibitions were observed on the mutagenesis induced by 4NQO, MNNG and B(a)P against TA100 strain. Methanol extracts from Kalopanacis cortex endothelium showed high antimutagenic effects against 4NQO, MNNG and B(a)P. The anticancer effects of methanol extract from Kalopanacis cortex endothelium against human lung carcinoma(A549), human breast adenocarcinoma(MCF-7) and human hepatocellular carcinoma(Hep3B) were investigated. The treatment of 0.5㎎/㎖ Kalopanacis cortex endothelium methanol extracts had the highest cytotoxicity against A549(81.54%), followed by MCF-7(81.92%) and Hep3B (78.57%). In contrast 0.5㎎/㎖ treatment of methanol extracts from Kalopanacis cortex endothelium had only 4~25% cytotoxicity on normal human liver cell(293).

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