Detrimental Effect of Bovine Serum Albumin in a Maturation Medium on Embryonic Development after Somatic Cell Nuclear Transfer in Pigs

초록

This study was designed to evaluate the effect of bovine serum albumin (BSA) in a maturation medium on oocytematuration and embryonic development in pigs. Immature pig oocytes were matured for 44 h in a medium supplementedwith 0.4% (w/v) BSA, 0.1% (w/v) polyvinyl alcohol (PVA), or 10% (v/v) pig follicular fluid (PFF). After IVM, oocytesreached metaphase II stage were activated for parthenogenesis (PA) or used as cytoplasts for somatic cell nucleartransfer (SCNT). Nuclear maturation (89.5%, 90.7% and 91.3% for BSA, PVA and PFF, respectively) and intraoocyteglutathione contents (1.20, 1.16 and 1.00 pixels/oocyte for BSA, PVA and PFF, respectively) were not altered by themacromolecules added to maturation medium. IVM of oocytes in a medium containing BSA (21.4%) and PVA (20.7%)showed significantly lower blastocyst formation after PA than culture in medium with PFF (39.2%). After SCNT,oocytes matured in medium with BSA showed decreased embryonic development to the blastocyst stage (9.2%)compared to those matured in medium with PFF (28.9%), while 23.6% of SCNT oocytes matured in medium withPVA developed to the blastocyst stage. When the effect of BSA in a maturation medium during the first 22 h andthe second 22 h of IVM in combination with PFF or PVA was examined, PVA-BSA showed a higher nuclearmaturation (94.1%) than BSA-PFF (84.5%). However, there was no significant difference in the blastocyst formationamong tested combinations (47.3, 52.2, 50.0, 44.4 and 49.0% for PFF-PFF, PFF-BSA, PVA-BSA, BSA-PVA andBSA-PFF, respectively). Our results demonstrate that BSA and PVA added to maturation medium can support oocytematuration comparable to PFF-supplemented medium. However, maturation of oocytes in a BSA-containing mediumdecreases embryonic development after PA and SCNT when compared with the medium supplemented with PFF.

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